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Image Search Results
Journal: bioRxiv
Article Title: OligoSeq: Rapid nanopore-sequencing of single-stranded oligonucleotides
doi: 10.1101/2025.06.10.658886
Figure Lengend Snippet: Using standard forward ( FW 20 ) and reverse ( RV 20 ) primers for exponential amplification of a single-stranded DNA template using PCR. The HiFi stands for High-Fidelity, high-temperature, DNA polymerase.
Article Snippet: A complementary strand of DNA can be either synthesised using commercially available DNA synthesisers, or by complementing the ssDNA by means of a
Techniques: Amplification
Journal: bioRxiv
Article Title: OligoSeq: Rapid nanopore-sequencing of single-stranded oligonucleotides
doi: 10.1101/2025.06.10.658886
Figure Lengend Snippet: A) Using standard reverse primers (RV 20 ), versus a random primer (non-amers, N 9 ) approach, for double-stranding complementation of ssDNA molecules enables B) ONT-adaptor compatibility and makes the double-stranded substrate suitable for ONT nanopore sequencing readout. HiFi stands for High-Fidelity, high-temperature, DNA polymerase (Q5 from NEB, UK) while PolI/Klen stands for low-temperature Klenow fragment of the DNA polymerase I.
Article Snippet: A complementary strand of DNA can be either synthesised using commercially available DNA synthesisers, or by complementing the ssDNA by means of a
Techniques: Nanopore Sequencing