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Image Search Results


Using standard forward ( FW 20 ) and reverse ( RV 20 ) primers for exponential amplification of a single-stranded DNA template using PCR. The HiFi stands for High-Fidelity, high-temperature, DNA polymerase.

Journal: bioRxiv

Article Title: OligoSeq: Rapid nanopore-sequencing of single-stranded oligonucleotides

doi: 10.1101/2025.06.10.658886

Figure Lengend Snippet: Using standard forward ( FW 20 ) and reverse ( RV 20 ) primers for exponential amplification of a single-stranded DNA template using PCR. The HiFi stands for High-Fidelity, high-temperature, DNA polymerase.

Article Snippet: A complementary strand of DNA can be either synthesised using commercially available DNA synthesisers, or by complementing the ssDNA by means of a DNA polymerase (which in this work can be either Q5 (Q5® High-Fidelity 2X Master Mix, NEB, UK) or the Klenow fragment (DNA Polymerase I, Large Klenow Fragment, NEB, UK); and a primer complementary to the reverse flanking end of the complemented DNA sequence (depicted as RV 20 , ), or a random nonamer (N9) randomly complementary to the reverse-complemented ssDNA (depicted as N 9 ).

Techniques: Amplification

A) Using standard reverse primers (RV 20 ), versus a random primer (non-amers, N 9 ) approach, for double-stranding complementation of ssDNA molecules enables B) ONT-adaptor compatibility and makes the double-stranded substrate suitable for ONT nanopore sequencing readout. HiFi stands for High-Fidelity, high-temperature, DNA polymerase (Q5 from NEB, UK) while PolI/Klen stands for low-temperature Klenow fragment of the DNA polymerase I.

Journal: bioRxiv

Article Title: OligoSeq: Rapid nanopore-sequencing of single-stranded oligonucleotides

doi: 10.1101/2025.06.10.658886

Figure Lengend Snippet: A) Using standard reverse primers (RV 20 ), versus a random primer (non-amers, N 9 ) approach, for double-stranding complementation of ssDNA molecules enables B) ONT-adaptor compatibility and makes the double-stranded substrate suitable for ONT nanopore sequencing readout. HiFi stands for High-Fidelity, high-temperature, DNA polymerase (Q5 from NEB, UK) while PolI/Klen stands for low-temperature Klenow fragment of the DNA polymerase I.

Article Snippet: A complementary strand of DNA can be either synthesised using commercially available DNA synthesisers, or by complementing the ssDNA by means of a DNA polymerase (which in this work can be either Q5 (Q5® High-Fidelity 2X Master Mix, NEB, UK) or the Klenow fragment (DNA Polymerase I, Large Klenow Fragment, NEB, UK); and a primer complementary to the reverse flanking end of the complemented DNA sequence (depicted as RV 20 , ), or a random nonamer (N9) randomly complementary to the reverse-complemented ssDNA (depicted as N 9 ).

Techniques: Nanopore Sequencing